Elisa Di Pasquale

Senior Researcher

Area of interest:

Dr. Di Pasquale research is mainly devoted to the understanding of the molecular basis of inherited cardiac diseases, i.e. arrhythmias and primary cardiomyopathies, a category of rare diseases caused by mutations in genes essential for the functioning of the heart. The ultimate goal of the laboratory is to boost the development of effective therapies for patients suffering from heart pathologies through the study of human cardiac cells generated from induced pluripotent stem cells (iPSC).

She graduated on Medical Biotechnologies in 2002 at the University of Milan, where she also obtained a PhD in Biotechnologies applied to Medical Sciences in 2005. After a post-doctoral training at the Rockefeller University in New York in the Laboratory of Molecular Vertebrate Embryology, she moved back to Italy and joined the Laboratory of Molecular Cardiology at IRCCS Multimedica Institute in Milan. From 2011, she holds a tenured position as researcher at the Milan Unit of the Institute of Genetic and Biomedical Research (IRGB) of the CNR. Currently, she is also co-head of the Stem Cells unit at Humanitas Research Hospital (Rozzano, Milan).

She gained years of experience in stem cell biology and investigation of mechanisms of human genetic diseases. Within the last 10 years, she developed iPSC-based models of inherited arrhythmias (i.e. Catecholaminergic Polymorphic Ventricular Tachycardia, long QT and Brugada syndromes) and primary cardiomyopathies that served as platforms to investigate the cellular and molecular events leading to the diseases and to test the efficacy of new therapeutic treatments.  More recently, she dedicated a particular effort to the understanding of the role of LMNA mutations in the onset and progression of dilated cardiomyopathy. A second research line relates to the mechanisms of cardiac differentiation and exploitation of bio-engineering approaches for the development of cardiac organoids/microtissues and for new therapeutic tools.

She received competitive grants from national and international agencies, as the Italian Ministry of Education University and Research, the Italian Ministry of Health and the European Community.

Most significant publications:


Crasto, S; My, I; Pasquale, E Di

Ŧhe Broad Spectrum of LMNA Cardiac Điseases: From Molecular Mechanisms to Clinical Phenotype Journal Article

In: Front Physiol, 11 , pp. 761, 2020.


Mazzola, M; Pasquale, E Di

Ŧoward Cardiac Regeneration: Combination of Pluripotent Stem Cell-Based Ŧherapies and Bioengineering Strategies Journal Article

In: Front Bioeng Biotechnol, 8 , pp. 455, 2020.



Lodola, F; Vurro, V; Crasto, S; Pasquale, E Di; Lanzani, G

Optical Pacing of Ħuman-Induced Pluripotent Stem Cell-Đerived Cardiomyocytes Mediated by a Conjugated Polymer Interface Journal Article

In: 8 (13), pp. e1900198, 2019.


Salvarani, N; Crasto, S; Miragoli, M; Bertero, A; Paulis, M; Kunderfranco, P; Serio, S; Forni, A; Lucarelli, C; Ferro, M Dal; Larcher, V; Sinagra, G; Vezzoni, P; Murry, C E; Faggian, G; Condorelli, G; Pasquale, E Di

Ŧhe K219Ŧ-Lamin mutation induces conduction defects through epigenetic inhibition of SCN5A in human cardiac laminopathy Journal Article

In: Nat Commun, 10 (1), pp. 2267, 2019.


Pasquale, E Di; Condorelli, G

Endoplasmic reticulum stress at the crossroads of progeria and atherosclerosis Journal Article

In: EMBO Mol Med, 11 (4), 2019.



Crasto, S; Pasquale, E Di

Induced Pluripotent Stem Cells to Study Mechanisms of Laminopathies: Focus on Epigenetics Journal Article

In: Front Cell Dev Biol, 6 , pp. 172, 2018.


Chatzifrangkeskou, M; Yadin, D; Marais, T; Chardonnet, S; Cohen-Tannoudji, M; Mougenot, N; Schmitt, A; Crasto, S; Pasquale, E Di; Macquart, C; Tanguy, Y; Jebeniani, I; Pucéat, M; Rodriguez, B Morales; Goldmann, W H; Ferro, M Dal; Biferi, M G; Knaus, P; Bonne, G; Worman, H J; Muchir, A

Cofilin-1 phosphorylation catalyzed by ERK1/2 alters cardiac actin dynamics in dilated cardiomyopathy caused by lamin A/C gene mutation Journal Article

In: Hum Mol Genet, 27 (17), pp. 3060–3078, 2018.



Kallikourdis, Marinos; Martini, Elisa; Carullo, Pierluigi; Sardi, Claudia; Roselli, Giuliana; Greco, Carolina M; Vignali, Debora; Riva, Federica; Berre, Anne Marie Ormbostad; Stølen, Tomas O; Fumero, Andrea; Faggian, Giuseppe; Pasquale, Elisa Di; Elia, Leonardo; Rumio, Cristiano; Catalucci, Daniele; Papait, Roberto; Condorelli, Gianluigi

T cell costimulation blockade blunts pressure overload-induced heart failure Journal Article

In: Nature Communications, 8 , pp. 14680, 2017, ISSN: 2041-1723.

Abstract | Links | BibTeX


Lodola, Francesco; Morone, Diego; Denegri, Marco; Bongianino, Rossana; Nakahama, Hiroko; Rutigliano, Lucia; Gosetti, Rosanna; Rizzo, Giulia; Vollero, Alessandra; Buonocore, Michelangelo; Napolitano, Carlo; Condorelli, Gianluigi; Priori, Silvia G; Pasquale, Elisa Di

Adeno-associated virus-mediated CASQ2 delivery rescues phenotypic alterations in a patient-specific model of recessive catecholaminergic polymorphic ventricular tachycardia Journal Article

In: Cell Death & Disease, 7 (10), pp. e2393, 2016, ISSN: 2041-4889.

Abstract | Links | BibTeX


Pasquale, E Di; Lodola, F; Miragoli, M; Denegri, M; Avelino-Cruz, J E; Buonocore, M; Nakahama, H; Portararo, P; Bloise, R; Napolitano, C; Condorelli, G; Priori, S G

CaMKII inhibition rectifies arrhythmic phenotype in a patient-specific model of catecholaminergic polymorphic ventricular tachycardia Journal Article

In: Cell Death & Disease, 4 , pp. e843, 2013, ISSN: 2041-4889.

Abstract | Links | BibTeX

Priori, Silvia G; Napolitano, Carlo; Pasquale, Elisa Di; Condorelli, Gianluigi

Induced pluripotent stem cell-derived cardiomyocytes in studies of inherited arrhythmias Journal Article

In: The Journal of Clinical Investigation, 123 (1), pp. 84–91, 2013, ISSN: 1558-8238.

Abstract | Links | BibTeX


Rizzi, R; Pasquale, E Di; Portararo, P; Papait, R; Cattaneo, P; Latronico, M V G; Altomare, C; Sala, L; Zaza, A; Hirsch, E; Naldini, L; Condorelli, G; Bearzi, C

Post-natal cardiomyocytes can generate iPS cells with an enhanced capacity toward cardiomyogenic re-differentation Journal Article

In: Cell Death and Differentiation, 19 (7), pp. 1162–1174, 2012, ISSN: 1476-5403.

Abstract | Links | BibTeX


Pasquale, E Di; Brivanlou, A H

Bone morphogenetic protein 15 (BMP15) acts as a BMP and Wnt inhibitor during early embryogenesis Journal Article

In: J Biol Chem, 284 (38), pp. 26127–26136, 2009.