Lucia Susani

Research Technician

Area of interest:

I am a Senior Technician expert in molecular and cell biology techniques. Responsible for the management of the cell culture database of our research group. 

In particular, in the last 10 years I have acquired a strong expertise in: mouse embryonic stem cell (ESCs) culture and characterization, mouse and human induced stem cells (iPSCs) culture and characterization, CRISPR-Cas9 gene-editing technique, microcell mediate chromosome transfer (MMCT) technique. 

Most significant publications:


Paulis, M; Susani, L; Castelli, A; Suzuki, T; Hara, T; Straniero, L; Duga, S; Strina, D; Mantero, S; Caldana, E; Sergi, L S; Villa, A; Vezzoni, P

Chromosome Ŧransplantation: A Possible Approach to Ŧreat Ħuman X-linked Đisorders Journal Article

In: Mol Ther Methods Clin Dev, 17 , pp. 369–377, 2020.



Castelli, A; Susani, L; Menale, C; Muggeo, S; Caldana, E; Strina, D; Cassani, B; Recordati, C; Scanziani, E; Ficara, F; Villa, A; Vezzoni, P; Paulis, M

Chromosome Ŧransplantation: Correction of the Chronic Granulomatous Đisease Đefect in Mouse Induced Pluripotent Stem Cells Journal Article

In: Stem Cells, 37 (7), pp. 876–887, 2019.



Susani, L; Castelli, A; Lizier, M; Lucchini, F; Vezzoni, P; Paulis, M

Correction of a Recessive Genetic Đefect by CRISPR-Cas9-Mediated Endogenous Repair Journal Article

In: CRISPR J, 1 , pp. 230–238, 2018.



Palagano, Eleonora; Susani, Lucia; Menale, Ciro; Ramenghi, Ugo; Berger, Massimo; Uva, Paolo; Oppo, Manuela; Vezzoni, Paolo; Villa, Anna; Sobacchi, Cristina

Synonymous Mutations Add a Layer of Complexity in the Diagnosis of Human Osteopetrosis Journal Article

In: Journal of Bone and Mineral Research: The Official Journal of the American Society for Bone and Mineral Research, 32 (1), pp. 99–105, 2017, ISSN: 1523-4681.

Abstract | Links | BibTeX


Paulis, Marianna; Castelli, Alessandra; Susani, Lucia; Lizier, Michela; Lagutina, Irina; Focarelli, Maria Luisa; Recordati, Camilla; Uva, Paolo; Faggioli, Francesca; Neri, Tui; Scanziani, Eugenio; Galli, Cesare; Lucchini, Franco; Villa, Anna; Vezzoni, Paolo

Chromosome transplantation as a novel approach for correcting complex genomic disorders Journal Article

In: Oncotarget, 6 (34), pp. 35218–35230, 2015, ISSN: 1949-2553.

Abstract | Links | BibTeX


Revenkova, E; Focarelli, M L; Susani, L; Paulis, M; Bassi, M T; Mannini, L; Frattini, A; Delia, D; Krantz, I; Vezzoni, P; Jessberger, R; Musio, A

Cornelia de Lange syndrome mutations in SMC1A or SMC3 affect binding to ĐNA Journal Article

In: Hum Mol Genet, 18 (3), pp. 418–427, 2009.



Susani, L; Pangrazio, A; Sobacchi, C; Taranta, A; Mortier, G; Savarirayan, R; Villa, A; Orchard, P; Vezzoni, P; Albertini, A; Frattini, A; Pagani, F

ŦCIRG1-dependent recessive osteopetrosis: mutation analysis, functional identification of the splicing defects, and in vitro rescue by U1 snRNA Journal Article

In: Hum Mutat, 24 (3), pp. 225–235, 2004.



Frattini, A; Pangrazio, A; Susani, L; Sobacchi, C; Mirolo, M; Abinun, M; Andolina, M; Flanagan, A; Horwitz, E M; Mihci, E; Notarangelo, L D; Ramenghi, U; Teti, A; Hove, J Van; Vujic, D; Young, T; Albertini, A; Orchard, P J; Vezzoni, P; Villa, A

Chloride channel ClCN7 mutations are responsible for severe recessive, dominant, and intermediate osteopetrosis Journal Article

In: J Bone Miner Res, 18 (10), pp. 1740–1747, 2003.



Zucchi, I; Bini, L; Albani, D; Valaperta, R; Liberatori, S; Raggiaschi, R; Montagna, C; Susani, L; Barbieri, O; Pallini, V; Vezzoni, P; Dulbecco, R

Đome formation in cell cultures as expression of an early stage of lactogenic differentiation of the mammary gland Journal Article

In: Proc Natl Acad Sci U S A, 99 (13), pp. 8660–8665, 2002.



Zucchi, I; Bini, L; Valaperta, R; Ginestra, A; Albani, D; Susani, L; Sanchez, J C; Liberatori, S; Magi, B; Raggiaschi, R; Hochstrasser, D F; Pallini, V; Vezzoni, P; Dulbecco, R

Proteomic dissection of dome formation in a mammary cell line: role of tropomyosin-5b and maspin Journal Article

In: Proc Natl Acad Sci U S A, 98 (10), pp. 5608–5613, 2001.



Zucchi, I; Montagna, C; Susani, L; Montesano, R; Affer, M; Zanotti, S; Redolfi, E; Vezzoni, P; Dulbecco, R

Genetic dissection of dome formation in a mammary cell line: identification of two genes with opposing action Journal Article

In: Proc Natl Acad Sci U S A, 96 (24), pp. 13766–13770, 1999.



Zucchi, I; Montagna, C; Susani, L; Vezzoni, P; Dulbecco, R

Ŧhe rat gene homologous to the human gene 9-27 is involved in the development of the mammary gland Journal Article

In: Proc Natl Acad Sci U S A, 95 (3), pp. 1079–1084, 1998.